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Aurion bsa-c incubation wash solution
Bsa C Incubation Wash Solution, supplied by Aurion, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bsa-c+solution/bsa+c+incubation+wash+solution/pm37776854-352-31-30
Average 90 stars, based on 1 article reviews
bsa-c incubation wash solution - by Bioz Stars, 2026-10
90/100 stars

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Saline:

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: Bacterial suspensions (optical density at 600 nm, 1) were mildly fixed with 0.5% paraformaldehyde, harvested by centrifugation, washed twice with PBS, incubated with each NCR-Fc (0.3 to 3 μg/ml) for 1 h at room temperature, washed twice in Aurion BSA-c solution (Wageningen, The Netherlands), and labeled with anti-human IgG (γ-chain specific) 10-nm-diameter-gold particle-conjugated antibody (diluted 1:20) (Sigma) for 45 min at room temperature.

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: After three washes in Aurion BSA-c solution, two washes in PBS, and a final wash in distilled water, a drop of suspension was placed onto a carbon-coated grid, partially dried, and directly observed without any further treatment.

Article Title: Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis.
Article Snippet: CD45– bone marrow erythroid cells (3 × 105 cells) were fixed with 4% paraformaldehyde and blocked with 1% BSA-C solution (Aurion) for 1 h. Subsequently, samples were permeabilized with 0.3% Triton X-100 in phosphate buffered saline and incubated with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals, Littleton, CO), then, subsequently a Cy5conjugated goat anti-rabbit IgG (H+L) F(ab’)2 fragments (Invitrogen, Carlsbad, CA).

Article Title: Immune surveillance properties of human NK cell-derived exosomes.
Article Snippet: RESEARCH ARTICLE | SEPTEMBER 15 2012 Immune Surveillance Properties of Human NK Cell-Derived Exosomes Luana Lugini; ... et. al J Immunol (2012) 189 (6): 2833–2842. https://doi.org/10.4049/jimmunol.1101988 Related Content Exosomes from B lymphocytes are a major source of Fas ligand J Immunol (May,2017) Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus J Immunol (May,2021) Exosomes secreted from virus- infected microglia can activate an in ammatory response in the central nervous system J Immunol (May,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/189/6/2833/1363747/1101988.pdf by guest on 15 D ecem ber 2024 The Journal of Immunology

Article Title: Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis
Article Snippet: CD45 – bone marrow erythroid cells (3 × 10 5 cells) were fixed with 4% paraformaldehyde and blocked with 1% BSA-C solution (Aurion) for 1 h. Subsequently, samples were permeabilized with 0.3% Triton X-100 in phosphate buffered saline and incubated with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals, Littleton, CO), then, subsequently a Cy5-conjugated goat anti-rabbit IgG (H+L) F(ab’) 2 fragments (Invitrogen, Carlsbad, CA).

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: After three washes in Aurion BSA-c solution, two washes in PBS, and a final wash in distilled water, a drop of suspension was placed onto a carbon-coated grid, partially dried, and directly observed without any further treatment.

Incubation:

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: Bacterial suspensions (optical density at 600 nm, 1) were mildly fixed with 0.5% paraformaldehyde, harvested by centrifugation, washed twice with PBS, incubated with each NCR-Fc (0.3 to 3 μg/ml) for 1 h at room temperature, washed twice in Aurion BSA-c solution (Wageningen, The Netherlands), and labeled with anti-human IgG (γ-chain specific) 10-nm-diameter-gold particle-conjugated antibody (diluted 1:20) (Sigma) for 45 min at room temperature.

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: After three washes in Aurion BSA-c solution, two washes in PBS, and a final wash in distilled water, a drop of suspension was placed onto a carbon-coated grid, partially dried, and directly observed without any further treatment.

Article Title: Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis.
Article Snippet: CD45– bone marrow erythroid cells (3 × 105 cells) were fixed with 4% paraformaldehyde and blocked with 1% BSA-C solution (Aurion) for 1 h. Subsequently, samples were permeabilized with 0.3% Triton X-100 in phosphate buffered saline and incubated with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals, Littleton, CO), then, subsequently a Cy5conjugated goat anti-rabbit IgG (H+L) F(ab’)2 fragments (Invitrogen, Carlsbad, CA).

Article Title: Immune surveillance properties of human NK cell-derived exosomes.
Article Snippet: RESEARCH ARTICLE | SEPTEMBER 15 2012 Immune Surveillance Properties of Human NK Cell-Derived Exosomes Luana Lugini; ... et. al J Immunol (2012) 189 (6): 2833–2842. https://doi.org/10.4049/jimmunol.1101988 Related Content Exosomes from B lymphocytes are a major source of Fas ligand J Immunol (May,2017) Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus J Immunol (May,2021) Exosomes secreted from virus- infected microglia can activate an in ammatory response in the central nervous system J Immunol (May,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/189/6/2833/1363747/1101988.pdf by guest on 15 D ecem ber 2024 The Journal of Immunology

Article Title: Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis
Article Snippet: CD45 – bone marrow erythroid cells (3 × 10 5 cells) were fixed with 4% paraformaldehyde and blocked with 1% BSA-C solution (Aurion) for 1 h. Subsequently, samples were permeabilized with 0.3% Triton X-100 in phosphate buffered saline and incubated with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals, Littleton, CO), then, subsequently a Cy5-conjugated goat anti-rabbit IgG (H+L) F(ab’) 2 fragments (Invitrogen, Carlsbad, CA).

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: After three washes in Aurion BSA-c solution, two washes in PBS, and a final wash in distilled water, a drop of suspension was placed onto a carbon-coated grid, partially dried, and directly observed without any further treatment.

Centrifugation:

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: Bacterial suspensions (optical density at 600 nm, 1) were mildly fixed with 0.5% paraformaldehyde, harvested by centrifugation, washed twice with PBS, incubated with each NCR-Fc (0.3 to 3 μg/ml) for 1 h at room temperature, washed twice in Aurion BSA-c solution (Wageningen, The Netherlands), and labeled with anti-human IgG (γ-chain specific) 10-nm-diameter-gold particle-conjugated antibody (diluted 1:20) (Sigma) for 45 min at room temperature.

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: After three washes in Aurion BSA-c solution, two washes in PBS, and a final wash in distilled water, a drop of suspension was placed onto a carbon-coated grid, partially dried, and directly observed without any further treatment.

Article Title: Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis.
Article Snippet: CD45– bone marrow erythroid cells (3 × 105 cells) were fixed with 4% paraformaldehyde and blocked with 1% BSA-C solution (Aurion) for 1 h. Subsequently, samples were permeabilized with 0.3% Triton X-100 in phosphate buffered saline and incubated with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals, Littleton, CO), then, subsequently a Cy5conjugated goat anti-rabbit IgG (H+L) F(ab’)2 fragments (Invitrogen, Carlsbad, CA).

Article Title: Immune surveillance properties of human NK cell-derived exosomes.
Article Snippet: RESEARCH ARTICLE | SEPTEMBER 15 2012 Immune Surveillance Properties of Human NK Cell-Derived Exosomes Luana Lugini; ... et. al J Immunol (2012) 189 (6): 2833–2842. https://doi.org/10.4049/jimmunol.1101988 Related Content Exosomes from B lymphocytes are a major source of Fas ligand J Immunol (May,2017) Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus J Immunol (May,2021) Exosomes secreted from virus- infected microglia can activate an in ammatory response in the central nervous system J Immunol (May,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/189/6/2833/1363747/1101988.pdf by guest on 15 D ecem ber 2024 The Journal of Immunology

Article Title: Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis
Article Snippet: CD45 – bone marrow erythroid cells (3 × 10 5 cells) were fixed with 4% paraformaldehyde and blocked with 1% BSA-C solution (Aurion) for 1 h. Subsequently, samples were permeabilized with 0.3% Triton X-100 in phosphate buffered saline and incubated with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals, Littleton, CO), then, subsequently a Cy5-conjugated goat anti-rabbit IgG (H+L) F(ab’) 2 fragments (Invitrogen, Carlsbad, CA).

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: After three washes in Aurion BSA-c solution, two washes in PBS, and a final wash in distilled water, a drop of suspension was placed onto a carbon-coated grid, partially dried, and directly observed without any further treatment.

Labeling:

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: Bacterial suspensions (optical density at 600 nm, 1) were mildly fixed with 0.5% paraformaldehyde, harvested by centrifugation, washed twice with PBS, incubated with each NCR-Fc (0.3 to 3 μg/ml) for 1 h at room temperature, washed twice in Aurion BSA-c solution (Wageningen, The Netherlands), and labeled with anti-human IgG (γ-chain specific) 10-nm-diameter-gold particle-conjugated antibody (diluted 1:20) (Sigma) for 45 min at room temperature.

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: After three washes in Aurion BSA-c solution, two washes in PBS, and a final wash in distilled water, a drop of suspension was placed onto a carbon-coated grid, partially dried, and directly observed without any further treatment.

Article Title: Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis.
Article Snippet: CD45– bone marrow erythroid cells (3 × 105 cells) were fixed with 4% paraformaldehyde and blocked with 1% BSA-C solution (Aurion) for 1 h. Subsequently, samples were permeabilized with 0.3% Triton X-100 in phosphate buffered saline and incubated with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals, Littleton, CO), then, subsequently a Cy5conjugated goat anti-rabbit IgG (H+L) F(ab’)2 fragments (Invitrogen, Carlsbad, CA).

Article Title: Immune surveillance properties of human NK cell-derived exosomes.
Article Snippet: RESEARCH ARTICLE | SEPTEMBER 15 2012 Immune Surveillance Properties of Human NK Cell-Derived Exosomes Luana Lugini; ... et. al J Immunol (2012) 189 (6): 2833–2842. https://doi.org/10.4049/jimmunol.1101988 Related Content Exosomes from B lymphocytes are a major source of Fas ligand J Immunol (May,2017) Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus J Immunol (May,2021) Exosomes secreted from virus- infected microglia can activate an in ammatory response in the central nervous system J Immunol (May,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/189/6/2833/1363747/1101988.pdf by guest on 15 D ecem ber 2024 The Journal of Immunology

Article Title: Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis
Article Snippet: CD45 – bone marrow erythroid cells (3 × 10 5 cells) were fixed with 4% paraformaldehyde and blocked with 1% BSA-C solution (Aurion) for 1 h. Subsequently, samples were permeabilized with 0.3% Triton X-100 in phosphate buffered saline and incubated with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals, Littleton, CO), then, subsequently a Cy5-conjugated goat anti-rabbit IgG (H+L) F(ab’) 2 fragments (Invitrogen, Carlsbad, CA).

Article Title: Direct Binding of Human NK Cell Natural Cytotoxicity Receptor NKp44 to the Surfaces of Mycobacteria and Other Bacteria
Article Snippet: After three washes in Aurion BSA-c solution, two washes in PBS, and a final wash in distilled water, a drop of suspension was placed onto a carbon-coated grid, partially dried, and directly observed without any further treatment.



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Inhibition of the RhoA/ROCK/MLC pathway restores the gate and fence functions of TJs in PSaV-infected polarized epithelial cells. Confluent LLC-PK cells grown on Transwell filters were untreated or treated with RhoA inhibitor CT04 (2 μg), ROCK inhibitor Y27632 (20 μM), or MLC inhibitor blebbistatin (10 μM) for 1 h at 37°C and then mock inoculated or inoculated with PSaV Cowden strain (MOI of 50) in the presence (A) or absence (B) of GCDCA for 5, 30, or 90 min. (A and B) Paracellular flux of FD4 and FD70 was measured in the apical-to-basolateral direction. The amount of FITC-dextran diffused to the basolateral side of the monolayer was normalized to the average obtained from control LLC-PK cells. As a positive control, confluent LLC-PK cells were treated for 10 min with 1.8 mM EGTA. All experiments were performed in triplicate. Data are presented as means ± standard errors from three independent experiments. Differences were evaluated using a one-way ANOVA. *, P < 0.05; **, P < 0.001; ***, P < 0.0001. (C and D) The distribution of the membrane fluorescent marker BodipyFL-C 12 -sphingomyelin-BSA (5 nmol/ml) loaded onto the apical surface of LLC-PK cells was determined via z-sectioning using a confocal microscope. All experiments were performed in triplicate; panels C and D show representative results.

Journal: Journal of Virology

Article Title: Porcine Sapovirus-Induced Tight Junction Dissociation via Activation of the RhoA/ROCK/MLC Signaling Pathway

doi: 10.1128/JVI.00051-21

Figure Lengend Snippet: Inhibition of the RhoA/ROCK/MLC pathway restores the gate and fence functions of TJs in PSaV-infected polarized epithelial cells. Confluent LLC-PK cells grown on Transwell filters were untreated or treated with RhoA inhibitor CT04 (2 μg), ROCK inhibitor Y27632 (20 μM), or MLC inhibitor blebbistatin (10 μM) for 1 h at 37°C and then mock inoculated or inoculated with PSaV Cowden strain (MOI of 50) in the presence (A) or absence (B) of GCDCA for 5, 30, or 90 min. (A and B) Paracellular flux of FD4 and FD70 was measured in the apical-to-basolateral direction. The amount of FITC-dextran diffused to the basolateral side of the monolayer was normalized to the average obtained from control LLC-PK cells. As a positive control, confluent LLC-PK cells were treated for 10 min with 1.8 mM EGTA. All experiments were performed in triplicate. Data are presented as means ± standard errors from three independent experiments. Differences were evaluated using a one-way ANOVA. *, P < 0.05; **, P < 0.001; ***, P < 0.0001. (C and D) The distribution of the membrane fluorescent marker BodipyFL-C 12 -sphingomyelin-BSA (5 nmol/ml) loaded onto the apical surface of LLC-PK cells was determined via z-sectioning using a confocal microscope. All experiments were performed in triplicate; panels C and D show representative results.

Article Snippet: Afterward, they were labeled on the apical side for 10 min on ice with a 5-nmol/ml solution of the BodipyFL-C 12 -sphingomyelin-BSA complex (Molecular Probes).

Techniques: Inhibition, Infection, Positive Control, Marker, Microscopy